C Vitamini ve Spektrofotometrik Yöntemle Tayini
Özet
Vücut için kritik bir antioksidan olan C vitamini, enzimatik fonksiyonlardan demir emilimine kadar pek çok hayati süreçte rol oynarken, eksikliği iskorbüt gibi ciddi hastalıklara yol açmaktadır. Gıda ve ilaç endüstrisindeki önemi nedeniyle, bu vitaminin miktarını belirlemek için kromatografi ve titrimetri gibi pek çok yöntem kullanılsa da, düşük maliyetli ve hızlı sonuç veren spektrofotometrik metotlar ön plana çıkmaktadır. Literatürdeki çalışmalar; meyve suları, ilaçlar ve biyolojik numunelerde C vitamini tayini için demir, bakır ve çeşitli boya reaktiflerine dayalı çok sayıda hassas ve özgün spektrofotometrik yöntemin başarıyla uygulandığını göstermektedir.
Vitamin C is an essential antioxidant for the human body that plays a key role in various biological processes, including enzymatic functions and iron absorption. Due to its widespread use in the food and pharmaceutical industries, numerous analytical methods have been developed for its determination, with spectrophotometric techniques being favored for their practicality, low cost, and speed. Current research highlights a variety of sensitive spectrophotometric methods using different reagents and reaction mechanisms to accurately measure vitamin C levels in products ranging from commercial fruit juices to biological samples.
Referanslar
Angulo C, Maldonado R, Pulgar et al. Vitamin C and oxidative stress in the seminiferous epithelium. Biological Research. 2011; 44(2), 169-180.
Padayatty SJ, Levine M. Vitamin C: the known and the unknown and Goldilocks. Oral Diseases. 2016; 22(6), 463-493.
Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition Journal. 2015; 15(1), 1-22.
Niki E. Interaction of ascorbate and alpha-tocopherol. Annals of the new York Academy of Sciences. 1987; 498, 186-199.
Singh P, Singh NP, Yadav RA. Study of the optimized molecular structures and vibrational characteristics of neutral L-Ascorbic acid and its anion and cation using density functional theory. Journal of Chemical and Pharmaceutical Research. 2010; 2(5), 656-681.
Yadav RA, Rani P, Kumar M, et al. Experimental IR and Raman spectra and quantum chemical studies of molecular structures, conformers and vibrational characteristics of L-ascorbic acid and its anion and cation. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2011; 84(1), 6-21.
Arya SP, Mahajan M, Jain P. Spectrophotometric determination of vitamin C with iron (II)-4-(2-pyridylazo) resorcinol complex. Analytica Chimica Acta. 2001; 427(2), 245-251.
Güçlü K, Sözgen K, Tütem E, et al. Spectrophotometric determination of ascorbic acid using copper (II)–neocuproine reagent in beverages and pharmaceuticals. Talanta. 2005; 65(5), 1226-1232.
Özyürek M, Güçlü K, Bektaşoğlu B, et al. Spectrophotometric determination of ascorbic acid by the modified CUPRAC method with extractive separation of flavonoids–La (III) complexes. Analytica chimica acta. 2007; 588(1), 88-95.
Tabata M, Morita H. Spectrophotometric determination of a nanomolar amount of ascorbic acid using its catalytic effect on copper (II) porphyrin formation. Talanta. 1997; 44(2), 151-157.
Besada A. A facile and sensitive spectrophotometric determination of ascorbic acid. Talanta. 1987; 34(8), 731-732.
Moeslinger T, Brunner M, Volf I, et al. Spectrophotometric determination of ascorbic acid and dehydroascorbic acid. Clinical chemistry. 1995; 41(8), 1177-1181.
Abdelmageed OH, Khashaba PY, Askal HF., et al. Selective spectrophotometric determination of ascorbic acid in drugs and foods. Talanta. 1995; 42(4), 573-579.
Selimović A, Salkić M, Selimović A. Direct spectrophotometric determination of L-ascorbic acid in pharmaceutical preparations using sodium oxalate as a stabilizer. International Journal of Basic and Applied Sciences. 2011; 11(2), 106-9.
Bazel Y, Riabukhina T, Tirpák J. Spectrophotometric determination of ascorbic acid in foods with the use of vortex-assisted liquid-liquid microextraction. Microchemical Journal. 2018; 143, 160-165.
Kleszczewski T, Kleszczewska E. Flow injection spectrophotometric determination of L-ascorbic acid in biological matters. Journal of Pharmaceutical and Biomedical Analysis. 2002; 29(4), 755-759.
Ensafi AA, Rezaei B, Movahedinia H. Kinetic–spectrophotometric determination of ascorbic acid by inhibition of the hydrochloric acid–bromate reaction. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2002; 58(12), 2589-2594.
Burns DT, Chimpalee N, Chimpalee D, et al. Flow-injection spectrophotometric determination of ascorbic acid by reduction of vanadotungstophosphoric acid. Analytica Chimica Acta. 1991; 243, 187-190.
Nejati‐Yazdinejad, M. Indirect determination of ascorbo Of Food Science & Technology. 2007; 42(12), 1402-1407.
Jagasia PV, Divyadarshani B. Spectrophotometric determination of vitamin C in pharmaceutical preparations using ammonium metavanadate. Asian Journal of Research in Chemistry. 20017; 10(3), 341-344.
Shrivas K, Agrawal K, Patel DK. A spectrophotometric determination of ascorbic acid. Journal of the Chinese Chemical Society. 2005; 52(3), 503-506.
Molina-Dıaz A, Ortega-Carmona I, Pascual-Reguera MI. Indirect spectrophotometric determination of ascorbic acid with ferrozine by flow-injection analysis. Talanta. 1998; 47(3), 531-536.
23 Shieh HH, Sweet TR. Spectrophotometric determination of ascorbic acid. Analytical Biochemistry. 1979; 96(1), 1-5.
Luque-Perez E, Rios A, Valcarcel M. Flow injection spectrophotometric determination of ascorbic acid in soft drinks and beer. Fresenius' Journal Of Analytical Chemistry. 2000; 366(8), 857-862.
Safavi A, Fotouhi L. Kinetic spectrophotometric determination of ascorbic acid by reduction of toluidine blue. Talanta. 1994; 41(8), 1225-1228.
