Modern Moleküler Sitogenetik
Özet
Ferzaneh Pordel Maragheh tarafından kaleme alınan ve Akademisyen Kitabevi bünyesinde yayımlanmış olan "Modern Moleküler Sitogenetik: Kısmı Bir Çalışma Kılavuzu" başlıklı bu eser, bitki sitogenetiği ve moleküler genom analizi alanında araştırmacılara rehberlik etmek amacıyla hazırlanmış son derece kapsamlı ve uygulamalı bir kaynak niteliğindedir. Yazarın Avrupa'daki akademik deneyimlerine dayanan kitap, özellikle Allium cinsi gibi kültür bitkilerinde gerçekleştirilen hücre kültürü, kromozom sayısı ve morfolojisinin tahmini ile karyotipleme çalışmalarına odaklanmaktadır. Eserde klasik ve modern sitogenetik yaklaşımlardan G-bantlama, C-bantlama, Feulgen boyama, aktif rRNA gen bölgelerinin tespiti için gümüş (NOR) boyama ve DAPI/CMA3+ çift boyama tekniklerinin prensipleri ile slayt hazırlama süreçleri adım adım açıklanmaktadır. Ayrıca 35S ve 5S rRNA genlerinin sayı ile kromozomal lokalizasyonlarını belirlemeye yarayan Floresan in situ Hibridizasyonu (FISH) yöntemi; prob hazırlığı, dNTP nükleotit etiketleme, denatürasyon, hibridizasyon, yıkama ve immüno-saptama adımlarıyla ayrıntılı olarak ele alınmaktadır. Laboratuvarda ihtiyaç duyulan tüm tampon ve solüsyonların reaktif tariflerinin yanı sıra parlak alan, faz kontrast, floresan, dijital görüntüleme, elektron mikroskobu ve akış sitometrisi gibi analiz cihazları ile görüntüleme yazılımlarına yer veren çalışma, moleküler biyoloji ve bitki ıslahı araştırmacıları için eksiksiz bir başvuru kılavuzu sunmaktadır.
Authored by Ferzaneh Pordel Maragheh and published by Akademisyen Kitabevi, the book titled "Modern Molecular Cytogenetics: A Partial Study Guide" serves as a highly comprehensive and practical resource designed to guide researchers in plant cytogenetics and molecular genome analysis. Drawing from the author's academic experiences in Europe, the book focuses on cell culture, estimation of chromosome number and morphology, and karyotyping studies conducted particularly on plant species of the genus Allium. The work explains step by step the principles and slide preparation procedures of classical and modern cytogenetic approaches, including G-banding, C-banding, Feulgen staining, silver (NOR) staining for detecting active rRNA gene regions, and DAPI/CMA3+ double staining. Furthermore, the Fluorescence In Situ Hybridization (FISH) technique used to determine the number and chromosomal localization of 35S and 5S rRNA genes is thoroughly detailed through probe preparation, dNTP nucleotide labeling, denaturation, hybridization, washing, and immunodetection steps. Featuring recipes for all buffers and solutions required in the laboratory alongside analysis devices such as brightfield, phase contrast, fluorescence, digital imaging, electron microscopy, flow cytometry, and image processing software, this work provides an indispensable reference guide for researchers in molecular biology and plant breeding.
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